m dapt  (TargetMol)

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 1. Time schedules for establishing the three groups (9 rats in each group). Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Control

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 3. Typical microscopic pathological images of HE and Masson staining in the lung tissues of rats in each group (n = 9). (a) HE staining (×100). In the control group, pulmonary alveoli, bronchioles and blood vessels were normal, and no changes in inflammation or fibrosis were observed. In the M group, the infiltration of interstitial inflammatory cells in the lung centered on the airway was remarkable, with proliferated fibrous connective tissue and noticeable proliferated fibrous tissue and smooth muscle tissue around the bronchioles and glands. The alveolar septum was widened with occasional multinucleated giant cells (indicated by a black arrow), and loose granuloma was formed. The inflammatory cell infiltration and fibrous connective tissue proliferation along the bronchus in the M+DAPT group were less severe than those in the M group. (b) Comparison of the collagen volume fractions (CVFs) was analyzed at a magnification of 40x using ImageJ software. CVF is the percentage of the blue area of collagen occupied in the total tissue area, and a higher CVF value indicates more severe fibrosis. (c) Masson staining (×100). Normal thin layers of connective tissue were observed under the submucosa of the bronchioles in the Ctrl group. In the M group, fibrosis of the bronchus and surrounding interstitial tissue was noticeable, and fibrosis developed around the granuloma in some areas (indicated by a yellow arrow). Fibrosis in the M+DAPT group was less severe than that in the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Staining, Control, Comparison, Software

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 4. Western blotting analysis of the ligands and receptors in the Notch pathway in the three groups (9 rats in each group). (a): Analysis of key Notch pathway protein levels. (b)-(g): Levels of the receptor and ligand proteins of the Notch signaling pathway. The letter a indicates p < 0.05 for the M group versus the control group, and the letter b indicates p < 0.05 for the M+DAPT group versus the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Western Blot, Control

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 5. The expression of the M1 macrophage marker genes TNF-α and iNOS and the M2 macrophage marker genes Arg-1 and Mrc2 in the three groups (9 rats in each group) according to PCR. The letter a indicates p < 0.05 for the M group versus the control group, and the letter b indicates p < 0.05 for the M+DAPT group versus the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Expressing, Marker, Control

Journal: European Journal of Inflammation

Article Title: Role of the Notch signaling pathway in regulating macrophage polarization in a rat model of fibrotic pigeon breeder’s lung

doi: 10.1177/1721727x231202431

Figure Lengend Snippet: Figure 6. Th1/Th2 ratio analysis of lung tissue in the three groups (9 rats in each) according to flow cytometry. The letter a indicates p < 0.05 for the M group versus the control group, and the letter b indicates p < 0.05 for the M+DAPT group versus the M group. Ctrl, control. M, model. M+DAPT, model+DAPT.

Article Snippet: The animals in the M+DAPT group received the same treatment as the M group, as well as DAPT (HY13027; MedChemExpress, New Jersey, US) at 0.05 mg/kg, which was administered intraperitoneally twice a week for four consecutive weeks followed by once a week until the 20th week.

Techniques: Cytometry, Control

Journal: BioMed Research International

Article Title: Hypoxia Enhances Fusion of Oral Squamous Carcinoma Cells and Epithelial Cells Partly via the Epithelial–Mesenchymal Transition of Epithelial Cells

doi: 10.1155/2018/5015203

Figure Lengend Snippet: (a) The expression of EMT-associated indexes including N-cadherin, E-cadherin, Vimentin, and Slug with/without hypoxia or DAPT stimulation. (b) The number of fused cells by artificial cell counting with/without the pretreated of hypoxia or DAPT for 3 days. (c) The fusion rate of the pretreated group and the control group by FACS. (i) Hypoxia (−), DAPT (−); (ii) hypoxia (−), DAPT (+); (iii) hypoxia (+), DAPT (−); (iv) hypoxia (+), DAPT (+). (d) Statistical analysis of fusion rate of the pretreated group and the control group ( ∗ p < 0.05).

Article Snippet: HIOECs were placed under hypoxia (1% O 2 ) or normoxia (5% CO 2 ) for 24 h; EMT was blocked by 5 μ M DAPT (Catalog number T6202, TargetMol, China).

Techniques: Expressing, Cell Counting

Journal: The Journal of Neuroscience

Article Title: Notch Suppression Collaborates with Ascl1 and Lin28 to Unleash a Regenerative Response in Fish Retina, But Not in Mice

doi: 10.1523/JNEUROSCI.2126-17.2018

Figure Lengend Snippet: Ascl1a and Lin28a expression synergize with Notch signaling inhibition to stimulate MG proliferation throughout the uninjured retina. A, Left, mCherry expression and GS immunofluorescence (green) in uninjured retinas of Tp1:mCherry fish and indicate that Notch signaling is confined to quiescent MG. Remaining panels show that MG proliferation (BrdU+; green signal) stimulated by forced Ascl1a and Lin 28 expression in the uninjured and injured retina of hsp70:ascl1a;hsp70:lin28a;Tp1:mCherry triple-transgenic fish is accompanied by reduced mCherry expression (red). Scale bar, 50 μm. B, BrdU immunofluorescence (red) shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) in the INL of uninjured retinas. Scale bar, 50 μm. C, Quantification of BrdU+ cells in Wt, hsp70:ascl1a, hsp70:lin28a, and hsp70:ascl1a;hsp70:lin28a fish treated with heat shock, ± DAPT or RO4929097; n = 3 different experiments. Error bars are SD. *p < 0.05, **p < 0.01, ***p < 0.001. D, BrdU immunofluorescence shows that forced Ascl1a and Lin28a expression synergizes with DAPT-treatment to stimulate MG proliferation (BrdU+) throughout the INL of uninjured retinas. Scale bar, 150 μm. E, qPCR quantification of her4.1 gene expression in uninjured retinas treated with DMSO, DAPT, or RO4929097; n = 3 different experiments. Error bars are SD. ***p < 0.001.

Article Snippet: For extended periods of heat shock, this was repeated every 6 h. To inhibit Notch signaling in fish, we immersed fish in water containing 40 μ m DAPT (Sigma-Aldrich) or RO4929097 (Cayman) prepared in DMSO and diluted 1/200 in fish water.

Techniques: Expressing, Inhibition, Immunofluorescence, Transgenic Assay